High-resolution fluorescence microscopy using three-dimensional structured illumination
نویسندگان
چکیده
We developed a high-resolution microscope based on three-dimensional structured illumination generated with two spatial light modulators. This setup enables both lateral resolution improvement by a factor two and axial localization of point like objects with nanometric precision.
منابع مشابه
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resolution, with true optical sectioning. A grating is used to generate three mutua...
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